restriction site

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restriction site

A scientist marks the restriction site on a DNA model.

Definition

Noun: A specific, short sequence of nucleotides in a DNA molecule that is recognized and cut by a particular restriction enzyme. It is the target location for enzymatic cleavage.

Usage

The term "restriction site" is used in molecular biology and genetics to describe the precise location on a DNA strand where a restriction endonuclease acts. It is a fundamental concept in techniques like DNA cloning, genetic engineering, and genome mapping.

Examples
  • The plasmid must contain a restriction site for EcoRI to insert the gene fragment.
  • Researchers analyzed the DNA sequence to identify all possible restriction sites.
  • The absence of a restriction site in the gene made the cloning strategy more complex.
Advanced Usage
  • "To map restriction sites": To determine the locations of restriction sites along a segment of DNA, creating a restriction map.
    • The first step was to map the restriction sites on the bacterial chromosome.
  • "Restriction site polymorphism": A variation in the DNA sequence that alters the presence or absence of a restriction site between individuals or populations.
    • The study focused on a restriction site polymorphism linked to the disease gene.
Variants and Related Words
  • Restriction Enzyme / Restriction Endonuclease (n): The enzyme that recognizes and cuts at a specific restriction site.
  • Restriction Fragment (n): A piece of DNA produced after cutting with a restriction enzyme.
  • Restriction Map (n): A diagram showing the locations of restriction sites within a DNA sequence.
Synonyms
  • Cleavage site
  • Recognition site (Note: This can be synonymous, though "recognition site" sometimes specifically refers to the sequence recognized, while "restriction site" implies the location of both recognition and cleavage.)
Related Phrases
  • "To cut at a restriction site": The action of the restriction enzyme cleaving the DNA.
    • The enzyme will cut at its restriction site, producing sticky ends.
  • "To contain/have a restriction site": Describes a DNA sequence that includes the specific nucleotide pattern.
    • Does this PCR product contain a restriction site for BamHI?
restriction site

A scientist marks the restriction site on a DNA model.

Noun
  1. the specific sites at which a restriction enzyme will cleave DNA